Gel electrophoresis

Samples of plasmid (pBR322) will be provided, precut with two restriction enzymes, along with a sample of a high-range DNA ladder to enable students to identify the fragments on their finished gel.

Each gel has enough wells to be used by two groups of students.

The gels take only 10 minutes to run, and the whole process can be completed in one period. However, we do recommend that you take two periods to use this equipment - one period discussing the technology and familiarising the students with the equipment and the protocols and a second period to run the gels.

Documents

• Gel electrophoresis presentation (PPT 8MB)
• Gel electrophoresis laboratory workbook (PDF, 2.7MB)
• Restriction enzyme worksheet
• Using a micropipette instruction sheet (PDF, 587KB)
• Running the gel instruction sheet (PDF, 510KB)
• An answer sheet will be supplied with the kits

Reservations

The kit is available to schools local to the University of Canterbury, the kit must be collected from and returned to UC by the school.

• Directions to UC Biological Sciences for collecting the kit (PDF, 332KB)

More information

For any enquiries regarding the kit, please contact Matt Walters.